PXD015269-1

PXD015269 is an original dataset announced via ProteomeXchange.

Title	High-resolution spatial proteome map of Toxoplasma gondii extracellular tachyzoite
Description	Apicomplexans, such as malaria-causing Plasmodium species, Toxoplasma, and Cryptosporidium, are parasitic protists that invade cells of virtually every animal, including humans. Despite the great burden on human healthcare and global economy, and the role in the environment, our understanding of the biology of these microorganisms is still very limited. Apicomplexans have evolved a complex cell architecture featuring a range of subcellular compartments, both common to all eukaryotes and unique to the phylum, that enable invasion into the host cell and exploiting its resources for growth and replication, rendering apicomplexans such remarkably successful parasites. In such an intricate subcellular landscape, protein function and location in the cell are tightly linked, which is why localising proteins in the cell is a major strategy in the apicomplexan research. Yet, the majority of proteins in model apicomplexans is unknown. In this project, a state-of-the-art spatial proteomics technology hyperLOPIT has been successfully adapted and applied to a model apicomplexan Toxoplasma gondii. Three independent hyperLOPIT experiments have been conducted on T. gondii strain RH extracellular tachyzoites, the invasive stage of the parasite's life cycle responsible for acute infection, providing the first cell-wide protein location atlas of any apicomplexan. This provides revolutionary insight into the apicomplexan cell organization and biology, including greatly expanded proteomes of secretory invasion organelles, the range of adaptive and regulatory responses of the organelles, and the subcellular distribution of evolutionary selection pressure, innovation, and conservation.
HostingRepository	PRIDE
AnnounceDate	2020-10-14
AnnouncementXML	Submission_2020-10-13_23:58:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD015269
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Konstantin Barylyuk
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-09-02 00:57:01	ID requested
⏵ 1	2020-10-13 23:58:57	announced
2	2020-10-21 23:57:58	announced	2020-10-22: Updated publication reference for PubMed record(s): 33053376.
3	2024-10-22 05:13:40	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Toxoplasma gondii, Apicomplexa, spatial, subcellular, sub-cellular, parasite, protist, localisation, localization, compartments, organelles, SPS-MS3, TMT10plex, machine learning

Ross F. Waller
contact affiliation	Department of Biochemistry, University of Cambridge, UK
contact email	rfw26@cam.ac.uk
lab head
Konstantin Barylyuk
contact affiliation	Department of Biochemistry, University of Cambridge
contact email	kb601@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD015269

PRIDE project URI

• PRIDE
  1. PXD015269
     1. Label: PRIDE project
     2. Name: High-resolution spatial proteome map of Toxoplasma gondii extracellular tachyzoite