PXD018397-1

PXD018397 is an original dataset announced via ProteomeXchange.

Title	Salinity adaptation in the principal arboviral vector Aedes aegypti characterized by larval cuticle changes
Description	Aedes aegypti, the principle global vector of arboviral diseases, has been widely regarded to only lay eggs and undergo preimaginal development in fresh water collections. Recent observations however show that it has adapted to develop in anthropogenic brackish water habitats of up to 50% sea water in coastal areas in different continents. This adaptation is characterised by greater salinity tolerance in adult oviposition preference, larvae and changes in sizes of anal papillae. The physiological basis for salinity tolerance in either Ae. aegypti or any of the known salinity-tolerant species of Anopheles malaria vectors is not established. To address this knowledge gap which is of fundamental biological interest and important for control of major diseases we performed RNAseq analysis of gut, anal papillae, and rest of the carcass of Ae. aegypti collected in the field from brackish water (BW) and fresh water habitats (FW) and then maintained as laboratory colonies in BW and FW respectively. We also examined the cuticle structure of larvae, pupae and adult BW and FW Ae. aegypti by microscopy and performed proteomic analysis of the shed cuticles of fourth instar larvae (L4) when they transformed into pupae. The results show that major changes in cuticle structure and composition characterize, and may be the principal factor that permits, the adaptation of Ae. aegypti to brackish water.
HostingRepository	PRIDE
AnnounceDate	2021-04-07
AnnouncementXML	Submission_2021-04-07_05:41:17.624.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD018397
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sebastien Voisin
SpeciesList	scientific name: Aedes aegypti; NCBI TaxID: 7159;
ModificationList	Amidated; Oxidation; Acetyl; Pyridylethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-06 02:16:36	ID requested
⏵ 1	2021-04-07 05:41:18	announced

Dataset with its publication pending

submitter keyword: Aedes aegypti, anal papillae structure, brackish water adaptation, larval cuticle proteins, gene expression, larval cuticle, mosquito vectors, salinity tolerance

Ranjan Ramasamy
contact affiliation	ID-FISH Technology Inc., Milpitas, CA 95035, USA
contact email	rramasamy@idfishtechnology.com
lab head
Sebastien Voisin
contact affiliation	Plateforme BioPark of Archamps
contact email	sebastien.voisin@biopark-archamps.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD018397
     1. Label: PRIDE project
     2. Name: Salinity adaptation in the principal arboviral vector Aedes aegypti characterized by larval cuticle changes