PXD019942-1

PXD019942 is an original dataset announced via ProteomeXchange.

Title	Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation
Description	TARGET OF RAPAMYCIN (TOR) is a deeply conserved protein kinase that coordinates eukaryotic metabolism with nutrient availability. In mammals, TOR specifically promotes translation of ribosomal protein mRNAs when amino acids are available to support protein synthesis. The mechanisms controlling translation downstream from TOR remain contested, however, and are largely unexplored in plants. Here, we took parallel global profiling approaches to define the in planta TOR-regulated proteome, and phosphoproteome. We found that TOR regulates ribosome biogenesis in plants at multiple levels. To investigate this further, we focused on a putative TOR substrate identified in our phosphoproteome: LARP1, a eukaryotic RNA-binding protein that is proposed to mediate TOR translational control of 5′TOP mRNAs in humans.
HostingRepository	PRIDE
AnnounceDate	2021-01-05
AnnouncementXML	Submission_2021-01-05_00:29:07.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD019942
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maria Regina Scarpin
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-22 03:09:54	ID requested
⏵ 1	2021-01-05 00:29:07	announced

Dataset with its publication pending

submitter keyword: Arabidopsis thaliana, seedlings, LC-MSMS

Jacob O. Brunkard
contact affiliation	Department of Plant and Microbial Biology, University of California at Berkeley, Berkeley, CA 94720, USA Plant Gene Expression Center, U.S. Department of Agriculture Agricultural Research Service, Albany, CA 94710, USA
contact email	brunkard@berkeley.edu
lab head
Maria Regina Scarpin
contact affiliation	Postdoctoral researcher
contact email	reginascarpin@berkeley.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD019942
     1. Label: PRIDE project
     2. Name: Parallel global profiling of plant TOR dynamics reveals a conserved role for LARP1 in protein translation