PXD023043-1

PXD023043 is an original dataset announced via ProteomeXchange.

Title	Elucidating the Cellular Machinery for Lipid Storage in Plants
Description	The overarching goal of this project is to understand the underlying mechanism for packaging storage lipids into lipid droplets in plant cells. Our group has identified several proteins involved in plant lipid droplet formation, but the inventory of proteins participating in this cellular process is far from complete. To search for new players involved in lipid droplet biogenesis and to gain more insights into how these players cooperate to package storage lipids into lipid droplets, we used an affinity capture approach coupled with mass spectrometry analysis to identify interactors of known lipid droplet-related proteins. Here, we used GFP-fused Arabidopsis thaliana LDIP and SEIPIN1 as baits to pull down their interactors in Nicotiana benthamiana leaves. The proteins that co-immunoprecipitated with GFP-LDIP and GFP-SEIPIN1 were analyzed by mass spectrometry and the results are shown here in this dataset.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_10:19:52.556.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023043
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yingqi Cai
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-10 01:35:50	ID requested
⏵ 1	2022-02-16 10:19:53	announced

Pyc M, Cai Y, Gidda SK, Yurchenko O, Park S, Kretzschmar FK, Ischebeck T, Valerius O, Braus GH, Chapman KD, Dyer JM, Mullen RT, Arabidopsis lipid droplet-associated protein (LDAP) - interacting protein (LDIP) influences lipid droplet size and neutral lipid homeostasis in both leaves and seeds. Plant J, 92(6):1182-1201(2017) [pubmed]

Pyc M, Gidda SK, Seay D, Esnay N, Kretzschmar FK, Cai Y, Doner NM, Greer MS, Hull JJ, Coulon D, Br, é, h, é, lin C, Yurchenko O, de Vries J, Valerius O, Braus GH, Ischebeck T, Chapman KD, Dyer JM, Mullen RT, LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis. Plant Cell, 33(9):3076-3103(2021) [pubmed]

submitter keyword: Lipid droplet, LDIP, SEIPIN, endoplasmic reticulum, neutral lipids

Kent D. Chapman
contact affiliation	BioDiscovery Institute, Department of Biological Sciences, University of North Texas
contact email	Kent.Chapman@unt.edu
lab head
Yingqi Cai
contact affiliation	University of North Texas
contact email	yingqicai@my.unt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023043

PRIDE project URI

• PRIDE
  1. PXD023043
     1. Label: PRIDE project
     2. Name: Elucidating the Cellular Machinery for Lipid Storage in Plants