PXD023401

PXD023401 is an original dataset announced via ProteomeXchange.

Title	Unbiased characterization and systematic profiling of metabolically labeled RNA-binding sites
Description	Here we introduce pRBS-ID, an amenable platform that enables robust profiling of PAR-crosslinked RBSs. Using tandem mass spectrometry (MS/MS) approaches, we unbiasedly characterize the PAR adducts on peptides, to develop an optimal MS/MS search pipeline that enables robust identification and quantification. Using pRBS-ID, we systematically profile RBSs crosslinked to U or G in PAR-labeled mRNA, which can serve as a rich resource for studying RNA-protein interactions. Especially, pRBS-ID is widely applicable to studies on time-course RNP remodelling, host-virus interactions, and RNP structure prediction.
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_04:14:57.301.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023401
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jong Woo Bae
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 04:14:47	ID requested
⏵ 1	2021-11-02 04:14:57	announced

Dataset with its publication pending

submitter keyword: Human, LC-MS/MS

Jong-Seo Kim
contact affiliation	School of Biological Sciences, Seoul National University, Seoul, Korea Center for RNA Research, Institute for Basic Science
contact email	jongseokim@snu.ac.kr
lab head
Jong Woo Bae
contact affiliation	Seoul National University
contact email	jakenizer@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD023401

PRIDE project URI

• PRIDE
  1. PXD023401
     1. Label: PRIDE project
     2. Name: Unbiased characterization and systematic profiling of metabolically labeled RNA-binding sites