PXD024796-1

PXD024796 is an original dataset announced via ProteomeXchange.

Title	Proximity biotinylation of CDK1 associated proteins in U2OS cells
Description	BirA ligase tagged CDK1 and BirA alone were expressed in U2OS cells. Proximal proteins to CDK1 were labelled by incubating cells with Biotin for 24 hours and biotinylated proteins extracted using Streptavidin pulldown.
HostingRepository	PRIDE
AnnounceDate	2021-05-12
AnnouncementXML	Submission_2021-05-11_22:44:06.733.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024796
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Jones
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 08:06:25	ID requested
⏵ 1	2021-05-11 22:44:07	announced

Dataset with its publication pending

submitter keyword: Human, CDK1, Proximity Biotinylation, LC-MSMS

Martin J Humphries
contact affiliation	University of Manchester
contact email	martin.humphries@manchester.ac.uk
lab head
Matthew Jones
contact affiliation	University of Manchester
contact email	matthew.jones-4@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024796

PRIDE project URI

• PRIDE
  1. PXD024796
     1. Label: PRIDE project
     2. Name: Proximity biotinylation of CDK1 associated proteins in U2OS cells