PXD026063-1

PXD026063 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis Wdr8 interactors identification
Description	To identify Arabidopsis Wdr8 interactors, immunoprecipitation associated LC-MS/MS analysis was carried out. Crude proteins extracted from Wdr8-GFP expressing transgenic plants was subjected to the immuno-precipitation assay using GFP antibody magnetic beads (µMACS GFP isolation kit, Miltenyi Biotec). Co-purified proteins were separated by 10% SDS-PAGE gel and stained with SYPRO Ruby (BioRad laboratories). The stained protein bands were excised into several fraction pieces according to protein sizes, and in-gel protein digestion by trypsin was carried out. Purified protein peptides were subjected to LC-MS/MS analysis (LTQ-Orbitrap XL-HTC-PAL system). Collected MS/MS peak spectra was analyzed by the MASCOT server to identify peptide sequence.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:41:46.921.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026063
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Noriyoshi Yagi
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-18 01:43:16	ID requested
⏵ 1	2022-02-17 03:41:48	announced

Yagi N, Kato T, Matsunaga S, Ehrhardt DW, Nakamura M, Hashimoto T, An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sites. Nat Commun, 12(1):3687(2021) [pubmed]

submitter keyword: Arabidopsis seedlings, GFP antibody coupled immunoprecipitation, LC-MS/MS

Takashi Hashimoto
contact affiliation	Division of Biological Science Nara Institute of Science and Technology
contact email	hasimoto@bs.naist.jp
lab head
Noriyoshi Yagi
contact affiliation	Institute of Transformative Bio-Molecules, Nagoya university
contact email	noriyoshi@itbm.nagoya-u.ac.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD026063

PRIDE project URI

• PRIDE
  1. PXD026063
     1. Label: PRIDE project
     2. Name: Arabidopsis Wdr8 interactors identification