PXD026895-1

PXD026895 is an original dataset announced via ProteomeXchange.

Title	Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros RS19
Description	Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaetoceros RS19, originally isolated from the Ross Sea, Antarctica, has a Zn requirement that cannot be met by Co and thus does not demonstrate a Zn/Co substitution ability as assessed by growth rate. We investigate the underlying metabolic basis of Zn/Co substitution at the transporter, sensor/chaperone, and metalloenzyme level by employing metal quota, metal uptake rate, and proteomic analyses of this substitution-incapable diatom. Co appears to be transported into the cell, but not efficiently utilized by Zn metalloenzymes.
HostingRepository	PRIDE
AnnounceDate	2022-03-02
AnnouncementXML	Submission_2022-03-02_10:02:15.647.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026895
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Riss Kellogg
SpeciesList	scientific name: Chaetoceros sp.; NCBI TaxID: 49240;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-23 22:18:40	ID requested
⏵ 1	2022-03-02 10:02:16	announced

Dataset with its publication pending

submitter keyword: zinc, cobalt, diatom, chaetoceros

Mak A. Saito
contact affiliation	Woods Hole Oceanographic Institution, Marine Chemistry and Geochemistry Department
contact email	msaito@whoi.edu
lab head
Riss Kellogg
contact affiliation	Woods Hole Oceanographic Institution/ Massachusetts Institute of Technology (WHOI-MIT)
contact email	mkell0gg@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD026895

PRIDE project URI

• PRIDE
  1. PXD026895
     1. Label: PRIDE project
     2. Name: Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros RS19