PXD027530 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Adsorptive microtiter plates as solid supports in affinity purification workflows |
Description | Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of captured protein from sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g. immunoassays) but hardly ever prior to LC-MS-based detection, and here we describe some applications, opportunities, and challenges of corresponding workflows. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-25 |
AnnouncementXML | Submission_2021-11-25_04:48:07.008.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD027530 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Horvatovich Péter |
SpeciesList | scientific name: Murine leukemia virus isolates; NCBI TaxID: 368798; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-25 14:31:05 | ID requested | |
⏵ 1 | 2021-11-25 04:48:07 | announced | |
Publication List
Klont F, Kwiatkowski M, Faiz A, van den Bosch T, Pouwels SD, Dekker FJ, Ten Hacken NHT, Horvatovich P, Bischoff R, Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows. J Proteome Res, 20(11):5218-5221(2021) [pubmed] |
Keyword List
submitter keyword: immuno capture, LC-MS/MS, Transcription factor p65, Peptidyl-prolyl cis-trans isomerase FKBP5 |
Contact List
Peter Horvatovich |
contact affiliation | University of Groningen |
contact email | p.l.horvatovich@rug.nl |
lab head | |
Horvatovich Péter |
contact affiliation | Faculty of Science and Engineering, Groningen Research Institute of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands |
contact email | p.l.horvatovich@rug.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027530
- Label: PRIDE project
- Name: Adsorptive microtiter plates as solid supports in affinity purification workflows