PXD027530-1

PXD027530 is an original dataset announced via ProteomeXchange.

Title	Adsorptive microtiter plates as solid supports in affinity purification workflows
Description	Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of captured protein from sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g. immunoassays) but hardly ever prior to LC-MS-based detection, and here we describe some applications, opportunities, and challenges of corresponding workflows.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_04:48:07.008.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027530
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Horvatovich Péter
SpeciesList	scientific name: Murine leukemia virus isolates; NCBI TaxID: 368798;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 14:31:05	ID requested
⏵ 1	2021-11-25 04:48:07	announced

Klont F, Kwiatkowski M, Faiz A, van den Bosch T, Pouwels SD, Dekker FJ, Ten Hacken NHT, Horvatovich P, Bischoff R, Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows. J Proteome Res, 20(11):5218-5221(2021) [pubmed]

submitter keyword: immuno capture, LC-MS/MS, Transcription factor p65, Peptidyl-prolyl cis-trans isomerase FKBP5

Peter Horvatovich
contact affiliation	University of Groningen
contact email	p.l.horvatovich@rug.nl
lab head
Horvatovich Péter
contact affiliation	Faculty of Science and Engineering, Groningen Research Institute of Pharmacy, Analytical Biochemistry, University of Groningen, 9713 AV Groningen, The Netherlands
contact email	p.l.horvatovich@rug.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027530
     1. Label: PRIDE project
     2. Name: Adsorptive microtiter plates as solid supports in affinity purification workflows