PXD030494-1

PXD030494 is an original dataset announced via ProteomeXchange.

Title	Baseline Proteomics Characterisation of Biomanufacturing Organism Halomonas Bluephagenesis
Description	Biomanufacturing remains financially uncompetitive with the lower cost but higher carbon emitting hydrocarbon based chemical industry. Novel chassis organisms may enable cost reductions with respect to traditional chassis such as E. coli and so open an economic rout to low emission biomanufacturing. Extremophile bacteria exemplify that potential. Salt tolerant halomonas species thrive in conditions inimical to other organisms. Their adoption would eliminate the cost of sterilising equipment. Novel chassis are inevitably poorly understood in comparison to established organisms. Rapid characterisation and community data sharing will facilitate organisms’ adoption for biomanufacturing. This paper describes baseline proteomics data set for Halomonas bluephagenesis TD01 under active development for biomanufactoring. The data record comprises a newly sequenced genome for the organism; evidence for expression of 1150 proteins (30% of the proteome) including baseline quantification of 1050 proteins (27% of the proteome) and a spectral library enabling re-use for targeted proteomics assays. Protein data is annotated with KEGG Orthology enabling rapid matching of quantitative data to pathways of interest to biomanufacturing.
HostingRepository	PRIDE
AnnounceDate	2022-07-11
AnnouncementXML	Submission_2022-07-11_08:43:21.071.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD030494
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew Russell
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Halomonas sp. TD01; NCBI TaxID: 999141;
ModificationList	iodoacetamide derivatized residue
Instrument	Xevo G2-S QTof

Revision	Datetime	Status	ChangeLog Entry
0	2021-12-17 13:21:00	ID requested
⏵ 1	2022-07-11 08:43:21	announced

Dataset with its publication pending

submitter keyword: Halomonas, Biomanufacturing, Biotechnology, Spectral Library

Nigel Scrutton
contact affiliation	Future Biomanufacturing Research Hub University of Manchester
contact email	nigel.scrutton@manchester.ac.uk
lab head
Matthew Russell
contact affiliation	University of Manchester
contact email	russell.matthew.r@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD030494

PRIDE project URI

• PRIDE
  1. PXD030494
     1. Label: PRIDE project
     2. Name: Baseline Proteomics Characterisation of Biomanufacturing Organism Halomonas Bluephagenesis