PXD031340-3

PXD031340 is an original dataset announced via ProteomeXchange.

Title	CpFTSY mediates co-translational insertion of thylakoid membrane proteins in diatoms, modulating their photosynthetic performances.
Description	CpFTSY is a component of the chloroplast signal recognition particle (CpSRP) pathway that post-translationally targets light-harvesting complex proteins (LHCPs) to the photosynthetic (thylakoid) membranes in plants and green algae containing chloroplasts derived from primary endosymbiosis. In plants, CpFTSY also plays a major role in co-translational incorporating of chloroplast encoded subunits of photosynthetic complexes to the thylakoids. This role has not been demonstrated in green algae. So far, its function in organisms with chloroplasts derived from secondary endosymbiotic events has not been elucidated. Here, we report the generation and characterization of mutants lacking CpFTSY in the diatom Phaeodactylum tricornutum. We found that this protein is not involved in inserting LHCPs into thylakoid membranes, indicating that the post-translational part of the CpSRP pathway is not active in this group of microalgae, consistent with earlier reports. However, a strong decline of the PsaC subunit of PSI indicates a role for CpFTSY in the co-translational CpSRP pathway in diatoms. Its absence leads to a very similar phenotype as the one previously described for diatoms lacking CpSRP54. Both cpftsy and cpsrp54 mutants display an increased level of photoprotection, low electron transport rates, inefficient repair of PSII, reduced growth and an upregulation of proteins compensating for a non-functional co-translational CpSRP pathway during light stress conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:55:36.014.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD031340
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Phaeodactylum tricornutum (strain CCAP 1055/1); NCBI TaxID: 556484;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-01-30 14:22:10	ID requested
1	2023-07-20 11:11:27	announced
2	2023-11-14 09:03:00	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:55:36	announced	2024-10-22: Updated project metadata.

10.1093/pcp/pcad014;

Nymark M, Finazzi G, Volpe C, Serif M, Fonseca DM, Sharma A, Sanchez N, Sharma AK, Ashcroft F, Kissen R, Winge P, Bones AM, Loss of CpFTSY Reduces Photosynthetic Performance and Affects Insertion of PsaC of PSI in Diatoms. Plant Cell Physiol, 64(6):583-603(2023) [pubmed]

10.6019/PXD031340;

submitter keyword: CpFTSY mediates co-translational insertion of thylakoid membrane proteins in diatoms, modulating their photosynthetic performances.

Marianne Nymark
contact affiliation	Associate professor II Department of Biology Faculty of Natural Sciences marianne.nymark@ntnu.no +4741528304 Realfagbygget, DU1-119, Gløshaugen, Høgskoleringen 5 Trondheim 7030 Norway
contact email	marianne.nymark@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD031340
     1. Label: PRIDE project
     2. Name: CpFTSY mediates co-translational insertion of thylakoid membrane proteins in diatoms, modulating their photosynthetic performances.