PXD033703-1

PXD033703 is an original dataset announced via ProteomeXchange.

Title	The proteomic and transcriptomic landscapes altered by Rgg2/3 activity in Streptococcus pyogenes
Description	Streptococcus pyogenes, otherwise known as Group A Streptococcus (GAS), is an important and highly adaptable human pathogen with the ability to cause both superficial and severe diseases. Understanding how S. pyogenes senses and responds to its environment will likely aid in determining how it causes a breadth of diseases. One regulatory network involved in GAS’s ability to sense and respond to the changing environment is the Rgg2/3 quorum sensing (QS) system, which responds to metal and carbohydrate availability and regulates changes to the bacterial surface. To better understand the impact of Rgg2/3 QS on S. pyogenes physiology, we performed RNA-seq and TMT-LC-MS/MS analysis on cells in which this system was induced or disrupted. Primary findings confirmed that pheromone stimulation in wildtype cultures is limited to the induction of operons whose promoters contain previously determined Rgg2/3 binding sequences. However, supplementing exogenous pheromone to a deletion mutant of rgg3, a strain that endogenously produces elevated amounts of pheromone, led to extended alterations of the transcriptome and proteome, ostensibly by stress-induced pathways. Under such exaggerated pheromone conditions (rgg3+SHP), a connection was identified between Rgg2/3 and the stringent response. Mutation of relA, the bifunctional guanosine tetra- and penta-phosphate nucleoside synthetase/hydrolase, and alarmone synthase genes sasA and sasB, impacted culture doubling times and disabled induction of Rgg2/3 in response to mannose, while manipulation of Rgg2/3 signaling modestly altered nucleotide levels. Our findings indicate that excessive pheromone production or exposure places stress on GAS resultingin an indirect altered proteome and transcriptome beyond primary pheromone signaling.
HostingRepository	PRIDE
AnnounceDate	2022-10-04
AnnouncementXML	Submission_2022-10-04_08:46:07.075.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD033703
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Britta Rued
SpeciesList	scientific name: Streptococcus pyogenes NZ131; NCBI TaxID: 471876;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-05 01:12:14	ID requested
⏵ 1	2022-10-04 08:46:07	announced
2	2023-11-14 08:56:25	announced	2023-11-14: Updated project metadata.

10.1128/JB.00175-22;

submitter keyword: Streptococcus pyogenes, Rgg2, Rgg3, quorum sensing

Michael Federle
contact affiliation	University of Illinois at Chicago
contact email	mfederle@uic.edu
lab head
Britta Rued
contact affiliation	University of Illinois at Chicago
contact email	brued@uic.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD033703
     1. Label: PRIDE project
     2. Name: The proteomic and transcriptomic landscapes altered by Rgg2/3 activity in Streptococcus pyogenes