PXD034400-1

PXD034400 is an original dataset announced via ProteomeXchange.

Title	PHF8 Demethylates YY1 to Regulate ETC Gene and mROS to Promote Cancer Cell Growth
Description	High levels of mitochondrial reactive oxygen species (mROS) are linked to cancer development, which is tightly controlled by electron transport chain (ETC). Targeting ETC represents a promising way of curing cancers. However, the epigenetic mechanisms governing ETC gene transcription to drive mROS production and cell growth remain to be fully characterized. Here, we report that, while it is well-known to demethylate histones to activate transcription, demethylase PHF8 also functions as a co-repressor for a large set of ETC genes via demethylating the transcription factor YY1 at a highly conserved lysine (K) residue, K258. Consequently, PHF8–mediated YY1 demethylation is critical for regulating mROS production and cell growth in multiple types of cancer cells as well as tumor growth in cell line-derived xenografts in vivo. Our data uncover a key epigenetic mechanism underlying ETC gene transcriptional regulation, demonstrating that targeting PHF8/YY1 axis has great potential to treat cancers.
HostingRepository	iProX
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2024-02-26_19:43:32.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaohui He
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	trimethylated residue; dimethylated residue; monomethylated residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-07 05:27:53	ID requested
⏵ 1	2024-02-26 19:43:33	announced

Wu XN, Li JY, He Q, Li BQ, He YH, Pan X, Wang MY, Sang R, Ding JC, Gao X, Wu Z, Liu W, Targeting the PHF8/YY1 axis suppresses cancer cell growth through modulation of ROS. Proc Natl Acad Sci U S A, 121(2):e2219352120(2024) [pubmed]

submitter keyword: PHF8, YY1, ETC, mROS

Wen Liu
contact affiliation	Xiamen University
contact email	w2liu@xmu.edu.cn
lab head
Yaohui He
contact affiliation	Xiamen University
contact email	yhhe@xmu.edu.cn
dataset submitter

iProX dataset URI