PXD039577-1

PXD039577 is an original dataset announced via ProteomeXchange.

Title	Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae
Description	Biofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for the human pathogen Vibrio cholerae, biofilm formation is not only a protective trait, but also an aggressive trait to collectively predate different types of immune cells. We find that V. cholerae forms biofilms on the eukaryotic cell surface using an extracellular matrix comprising primarily mannose-sensitive hemagglutinin pili, toxin-coregulated pili, and the secreted colonization factor TcpF, which is distinct from the matrix composition of biofilms on abiotic surfaces. These biofilms encase immune cells and establish a high local concentration of a secreted hemolysin to kill the immune cells, before the biofilms disperse in a c-di-GMP-dependent manner. Together, these results uncover a mechanism for how bacteria employ biofilm formation as a multicellular strategy to invert the typical relationship between the human immune system as the hunter and bacteria as the hunted.
HostingRepository	PRIDE
AnnounceDate	2023-05-25
AnnouncementXML	Submission_2023-05-25_13:02:10.455.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD039577
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	ThomasBock
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-01-20 02:38:38	ID requested
⏵ 1	2023-05-25 13:02:10	announced
2	2023-11-14 09:01:08	announced	2023-11-14: Updated project metadata.

10.6019/PXD039577;

submitter keyword: Vibrio cholerae,Biofilm formation

AlexanderSchmidt
contact affiliation	Alexander Schmidt, Biozentrum, University of Basel Spitalstrasse 41, CH-4056 Basel, Switzerland Tel. +41 61 207 0927, https://www.biozentrum.unibas.ch
contact email	alex.schmidt@unibas.ch
lab head
ThomasBock
contact affiliation	Proteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact email	thomas.bock@unibas.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD039577

PRIDE project URI

• PRIDE
  1. PXD039577
     1. Label: PRIDE project
     2. Name: Biofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae