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PXD039577 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBiofilm formation on human immune cells is a multicellular predation strategy of Vibrio cholerae
DescriptionBiofilm formation is generally recognized as a bacterial defense mechanism against environmental threats, including antibiotics, bacteriophages, and leukocytes of the human immune system. Here, we show that for the human pathogen Vibrio cholerae, biofilm formation is not only a protective trait, but also an aggressive trait to collectively predate different types of immune cells. We find that V. cholerae forms biofilms on the eukaryotic cell surface using an extracellular matrix comprising primarily mannose-sensitive hemagglutinin pili, toxin-coregulated pili, and the secreted colonization factor TcpF, which is distinct from the matrix composition of biofilms on abiotic surfaces. These biofilms encase immune cells and establish a high local concentration of a secreted hemolysin to kill the immune cells, before the biofilms disperse in a c-di-GMP-dependent manner. Together, these results uncover a mechanism for how bacteria employ biofilm formation as a multicellular strategy to invert the typical relationship between the human immune system as the hunter and bacteria as the hunted.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-01-20 02:38:38ID requested
12023-05-25 13:02:10announced
Publication List
Keyword List
submitter keyword: Vibrio cholerae,Biofilm formation
Contact List
contact affiliationAlexander Schmidt, Biozentrum, University of Basel Spitalstrasse 41, CH-4056 Basel, Switzerland Tel. +41 61 207 0927, https://www.biozentrum.unibas.ch
contact emailalex.schmidt@unibas.ch
lab head
contact affiliationProteomics Core Facility, Biozentrum, University of Basel, Switzerland
contact emailthomas.bock@unibas.ch
dataset submitter
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Dataset FTP location
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