PXD040520-1

PXD040520 is an original dataset announced via ProteomeXchange.

Title	The proteomic response of C. elegans to two protective Pseudomonas symbionts
Description	The two C. elegans natural microbiota isolates Pseudomonas lurida MYb11 and Pseudomonas fluorescens MYb115 protect the host against pathogens through distinct mechanisms. While P. lurida produces an antimicrobial compound and directly inhibits pathogen growth, P. fluorescens MYb115 protects the host without affecting pathogen growth. It is unknown how these two protective microbes affect host biological processes. We used a proteomics approach to elucidate the C. elegans response to MYb11 and MYb115. We found that both Pseudomonas isolates increase vitellogenin protein production in adult day 1 animals, which confirms previous findings on the effect of microbiota on C. elegans reproductive timing. Moreover, the C. elegans responses to MYb11 and MYb115 exhibit common signatures with the response to other vitamin B12-producing bacteria, emphasizing the importance of vitamin B12 in C. elegans-microbe metabolic interactions.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:34:02.116.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-01 03:51:36	ID requested
⏵ 1	2024-05-24 01:34:03	announced
2	2024-10-22 06:42:25	announced	2024-10-22: Updated project metadata.

10.1128/mbio.03463-23;

Pees B, Peters L, Treitz C, Hamerich IK, Kissoyan KAB, Tholey A, Dierking K, symbionts. mBio, 15(4):e0346323(2024) [pubmed]

submitter keyword: C. elegans, Microbiota, Host-Symbiont interactions

Andreas Tholey
contact affiliation	Institut für Experimentelle Medizin (IEM) Arbeitsgruppe Systematische Proteomforschung Christian-Albrechts-Universität zu Kiel Niemannsweg 11 (Campus UK-SH, Kiel) 24105 Kiel, Germany
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD040520

PRIDE project URI

• PRIDE
  1. PXD040520
     1. Label: PRIDE project
     2. Name: The proteomic response of C. elegans to two protective Pseudomonas symbionts