PXD040594-1

PXD040594 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomics of detergent-solubilized membrane proteins from Trypanosoma evansi
Description	Trypanosoma evansi, the etiological agent of Surra, is the most widespread pathogenic salivarian trypanosomes and affects most livestock and wild animals in endemic regions. Knowledge of the molecular and cell biology of the parasite is limited. The study of proteins through a proteomic-based approach contributes to biochemical, molecular, and epidemiological studies. In this work, high-throughput proteomic analysis of Triton X-114-extracted surface-enriched proteins of bloodstream forms of T. evansi was carried out by gel-free approach (LC-ESI-MS/MS). Proteins resolved by SDS-PAGE revealed major proteins bands between 35 kDa and 100 kDa. After MS analysis of three biological and technical replicates, 385 proteins were identified. From these proteins, xx (0.77 %) were predicted to have GPI-anchor, 137 (35.5 %) were predicted to have transmembrane domains by (TMHMM), and 154 (40 %) were predicted as membrane-bound proteins. Membrane proteins typical of each organelle were found, some of which were already studied in other trypanosomatids. Two proteins, a and b, were considered T. evansi singletons, one of them, a, with two predicted epitopes, a putative 75 kDa invariant surface glycoprotein.
HostingRepository	PRIDE
AnnounceDate	2024-07-03
AnnouncementXML	Submission_2024-07-03_02:01:01.628.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD040594
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Renato Moreira
SpeciesList	scientific name: Trypanosoma evansi; NCBI TaxID: 5697;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-04 14:16:47	ID requested
⏵ 1	2024-07-03 02:01:02	announced

10.6019/PXD040594;

Batista F, Moreira RS, Filho VB, Moura H, Wagner G, Miletti LC, Shotgun proteomics of detergent-solubilized proteins from Trypanosoma evansi. J Proteomics, 304():105231(2024) [pubmed]

10.1016/j.jprot.2024.105231;

submitter keyword: proteomic, Triton-X114,Surface proteins

Luiz Claudio Miletti
contact affiliation	Laboratório de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC). Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000 Brazil.
contact email	luiz.miletti@udesc.br
lab head
Renato Moreira
contact affiliation	Federal Institute of Santa Catarina
contact email	renatosm@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040594

PRIDE project URI

• PRIDE
  1. PXD040594
     1. Label: PRIDE project
     2. Name: Shotgun proteomics of detergent-solubilized membrane proteins from Trypanosoma evansi