PXD040811-2

PXD040811 is an original dataset announced via ProteomeXchange.

Title	Multilevel proteomics reveals common and specific host perturbations by Monkeypox and Vaccinia viruses (full proteome)
Description	The global spread of Monkeypox virus (MPXV) has resulted in the urgent need for an in-depth molecular characterization of the virus infection. Multiple omics studies on Vaccinia virus have extended our knowledge of poxvirus pathophysiology (REFs). Nevertheless, there are no comparative studies that would include an in-depth comparison of MPXV with other poxviruses in the context of molecular biology. Here, we report a comparative time-resolved proteomic study of MPXV and vaccinia viruses and a concurrent multi-omics study of MPXV infection in primary human cells. Using state-of-the-art proteomics, we profiled the virus-host interplay on the transcriptome, proteome and phosphoproteome levels in primary human foreskin fibroblasts. Pathway analysis in combination with projecting the gathered data onto the global network of host-signaling interactions revealed crosstalk between the perturbations at different levels, enabling identification of distinct and common molecular mechanisms of poxviruses. The NF-κB pathway, a signaling pathway governing immunomodulation and inflammation, was unexpectedly activated by MPXV but not VacV, while MPXV exhibited an astounding resistance to the effects of antiviral interferon response, potentially explaining the unique pathogenicity hallmarks of the monkeypox. Our extensive dataset highlights many signaling events and hotspots that influence the MPXV life cycle and may be used to guide rational design of both virus- and host-directed therapies, which we exemplify by identifying inhibitors of XXX, YYY, and ZZZ with potent antiviral efficacy against MPXV and/or VacV.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:51:30.121.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quirin Emslander
SpeciesList	scientific name: Monkeypox virus; NCBI TaxID: NCBITaxon:10244; scientific name: Monkeypox virus; NCBI TaxID: 10244; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Orthopoxvirus; NCBI TaxID: NCBITaxon:10242;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-13 07:43:58	ID requested
1	2024-07-25 10:36:07	announced
⏵ 2	2024-10-22 06:51:30	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human, Poxvirus, Shotgun-proteomics, Vaccinia Virus, Monkeypox virus

Andreas Pichlmair
contact affiliation	Prof. Andreas Pichlmair, Technical University Munich, Faculty of Medicine, Institute of Virology, Viral Immunopathology, Schneckenburger Str. 8, D-81675 Munich, Germany
contact email	andreas.pichlmair@tum.de
lab head
Quirin Emslander
contact affiliation	TUM
contact email	quirin.emslander@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD040811

PRIDE project URI

• PRIDE
  1. PXD040811
     1. Label: PRIDE project
     2. Name: Multilevel proteomics reveals common and specific host perturbations by Monkeypox and Vaccinia viruses (full proteome)