PXD041204-1

PXD041204 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Hydrogen deuterium exchange epitope mapping of glycosylated epitopes enabled by online immobilized glycosidase
Description	Numerous mAbs are known to recognize N-glycosylated epitopes or to bind in close proximity to an N-glycan site, however, glycosylated protein sites are typically obscured from HDX detection as a result of the inherent heterogeneity of glycans. To overcome this limitation, we covalently immobilized the glycosidase PNGase Dj on solid resin and incorporated it into an online HDX-MS workflow for post-HDX deglycosylation. The resin-immobilized PNGase Dj exhibits a robust tolerance to various buffer conditions, and is employed in a column format that can be readily adapted into a typical HDX-MS platform. Using this system, we were able to obtain full sequence coverage of the SARS-CoV-2 receptor binding domain (RBD) and to map the glycosylated epitope of the glycan-binding mAb S309 to the RBD.
HostingRepository	PRIDE
AnnounceDate	2024-05-24
AnnouncementXML	Submission_2024-05-24_01:28:06.023.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timothy O'Leary
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-03-30 03:35:28	ID requested
⏵ 1	2024-05-24 01:28:06	announced

Publication List

O'Leary TR, Balasubramaniam D, Hughes K, Foster D, Boyles J, Coleman K, Griffin PR, Hydrogen-Deuterium Exchange Epitope Mapping of Glycosylated Epitopes Enabled by Online Immobilized Glycosidase. Anal Chem, 95(27):10204-10210(2023) [pubmed]
10.1021/acs.analchem.3c00374;

O'Leary TR, Balasubramaniam D, Hughes K, Foster D, Boyles J, Coleman K, Griffin PR, Hydrogen-Deuterium Exchange Epitope Mapping of Glycosylated Epitopes Enabled by Online Immobilized Glycosidase. Anal Chem, 95(27):10204-10210(2023) [pubmed]

10.1021/acs.analchem.3c00374;

Keyword List

submitter keyword: LC/MS-MS,HDX

submitter keyword: LC/MS-MS,HDX

Contact List

Patrick R. Griffin
contact affiliation	Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, Jupiter, FL 33458, USA
contact email	pgriffin2@ufl.edu
lab head
Timothy O'Leary
contact affiliation	The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
contact email	olearytimothy@ufl.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD041204
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD041204

PRIDE project URI

Repository Record List

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