PXD044653-1

PXD044653 is an original dataset announced via ProteomeXchange.

Title	Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (4)
Description	mRNA degradation is one of the main steps of gene expression, and a key player is the 5’-3’ exonuclease Xrn1. In Saccharomyces cerevisiae, it was previously shown, by a microscopy approach, that Xrn1 is located to different cellular compartments, depending on physiological state. During exponential growth, Xrn1 is distributed in the cytoplasm, while it is present in the eisosomes after the post-diauxic shift (PDS). Here, we biochemically characterized the Xrn1-associated complexes in different cellular states. We demonstrate that, after PDS, Xrn1 but not the decapping (DCP), nor Lsm1-7/Pat1 complexes, was sequestered in the eisosomes, thus preserving mRNAs from degradation.
HostingRepository	PRIDE
AnnounceDate	2023-09-15
AnnouncementXML	Submission_2023-09-15_06:29:04.660.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD044653
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	EmmanuellePermal
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-08-18 09:23:04	ID requested
⏵ 1	2023-09-15 06:29:05	announced
2	2024-10-22 06:03:11	announced	2024-10-22: Updated project metadata.

10.6019/PXD044653;

submitter keyword: Affinity purification,Xrn1, post diauxic shift, eisosome, Saccharomyces cerevisiae

MichelineFromont-Racine
contact affiliation	Cytoplasmic mRNA surveillance in yeast DEPARTMENT OF GENOMES AND GENETICS, Institut Pasteur
contact email	micheline.fromont-racine@pasteur.fr
lab head
EmmanuellePermal
contact affiliation	Institut Pasteur
contact email	emmanuelle.permal@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD044653

PRIDE project URI

• PRIDE
  1. PXD044653
     1. Label: PRIDE project
     2. Name: Xrn1 biochemically associates to the eisosome after the post diauxic shift in yeast (4)