PXD050288-2

PXD050288 is an original dataset announced via ProteomeXchange.

Title	An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway
Description	Animals sense and respond to nutrient availability in their environments, a task coordinated in part by the mTOR complex 1 (mTORC1) pathway. mTORC1 regulates growth in response to nutrients and, in mammals, senses specific amino acids through specialized sensors that bind the GATOR1/2 signaling hub. Given that animals can occupy diverse niches, we hypothesized that the pathway might evolve distinct sensors in different metazoan phyla. Whether such customization occurs—and how the mTORC1 pathway might capture new inputs—is unknown. Here, we identify the Drosophila melanogaster protein Unmet expectations (CG11596) as a species-restricted methionine sensor that directly binds the fly GATOR2 complex in a fashion antagonized by S-adenosylmethionine (SAM). We find that in Dipterans GATOR2 rapidly evolved the capacity to bind Unmet and to thereby repurpose a previously independent methyltransferase as a SAM sensor. Thus, the modular architecture of the mTORC1 pathway allows it to co-opt preexisting enzymes to expand its nutrient sensing capabilities, revealing a mechanism for conferring evolvability on an otherwise conserved system.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:34:23.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Grace Liu
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-03 13:29:00	ID requested
1	2024-03-21 15:46:46	announced
⏵ 2	2024-10-22 06:34:24	announced	2024-10-22: Updated project metadata.

10.1038/S41467-024-46680-3;

submitter keyword: fly, TORC1, nutrient sensor, Drosophila,mTORC1

David M. Sabatini
contact affiliation	DMS: Institute of Organic Chemistry and Biochemistry; Prague, Czech Republic
contact email	david.sabatini@uochb.cas.cz
lab head
Grace Liu
contact affiliation	University of California, Berkeley
contact email	grace.yun.liu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/03/PXD050288

PRIDE project URI

• PRIDE
  1. PXD050288
     1. Label: PRIDE project
     2. Name: An evolutionary mechanism to assimilate new nutrient sensors into the mTORC1 pathway