PXD050601-2

PXD050601 is an original dataset announced via ProteomeXchange.

Title	Regulation by the RNA-binding protein Unkempt at its effector interface
Description	How RNA-binding proteins (RBPs) convey regulatory instructions to the core effectors of RNA processing is unclear. Here we document the existence and functions of a multivalent RBP–effector interface. We show that the effector interface of a conserved RBP with an essential role in metazoan development, Unkempt, is mediated by a novel type of ‘dual-purpose’ peptide motifs that can contact two different surfaces of interacting proteins. Unexpectedly, we find that the multivalent contacts do not merely serve effector recruitment but are required for the accuracy of RNA recognition by Unkempt. Systems analyses reveal that multivalent RBP–effector contacts can repurpose the principal activity of an effector for a different function, as we demonstrate for reuse of the central eukaryotic mRNA decay factor CCR4-NOT in translational control. Our study establishes the molecular assembly and functional principles of an RBP–effector interface.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:35:54.089.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD050601
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shiyang He
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-03-13 15:07:02	ID requested
1	2024-04-11 13:49:14	announced
⏵ 2	2024-10-22 06:35:54	announced	2024-10-22: Updated project metadata.

10.6019/PXD050601;

10.1038/S41467-024-47449-4;

submitter keyword: UNK, RNA binding protein

Jernej Murn
contact affiliation	University of California, Riverside
contact email	murnj@ucr.edu
lab head
Shiyang He
contact affiliation	UC Riverside
contact email	she050@ucr.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD050601

PRIDE project URI

• PRIDE
  1. PXD050601
     1. Label: PRIDE project
     2. Name: Regulation by the RNA-binding protein Unkempt at its effector interface