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PXD007956

PXD007956 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLinking microbial community structure and function during the anaerobic digestion of grass
DescriptionIn this study, microbial communities from triplicate leach-bed anaerobic bioreactors digesting grass were analysed. Each reactor comprised two microbial fractions, one immobilized on grass (biofilm) and the other in a planktonic state present in the leachate. Microbial communities from the two fractions were systematically investigated for community composition and function. This was carried out using DNA, RNA and protein co-extraction. The microbial structure of each fraction was examined using 16S rRNA deep sequencing, while the active members of the consortia were identified using the same approach on cDNA generated from co-extracted RNA samples. Microbial function was investigated using a metaproteomic workflow combining SDS-PAGE and LC-MS/MS analysis.
HostingRepositoryPRIDE
AnnounceDate2018-10-23
AnnouncementXMLSubmission_2019-03-07_05:42:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAoife Joyce
SpeciesList scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-10-12 03:52:42ID requested
12017-12-07 05:50:15announced
22019-03-07 05:42:11announcedUpdated publication reference for PubMed record(s): 29619022.
Publication List
Joyce A, Ijaz UZ, Nzeteu C, Vaughan A, Shirran SL, Botting CH, Quince C, O'Flaherty V, Abram F, Linking Microbial Community Structure and Function During the Acidified Anaerobic Digestion of Grass. Front Microbiol, 9():540(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Anaerobic digestion
Metaproteomics
Grass
Contact List
Florence Abram
contact affiliationFunctional Environmental Microbiology, School of Natural Sciences, National University of Ireland Galway
contact emailflorence.abram@nuigalway.ie
lab head
Aoife Joyce
contact affiliationNUI Maynooth
contact emailfeefeeseoighe@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
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