PXD007956-1

PXD007956 is an original dataset announced via ProteomeXchange.

Title	Linking microbial community structure and function during the anaerobic digestion of grass
Description	In this study, microbial communities from triplicate leach-bed anaerobic bioreactors digesting grass were analysed. Each reactor comprised two microbial fractions, one immobilized on grass (biofilm) and the other in a planktonic state present in the leachate. Microbial communities from the two fractions were systematically investigated for community composition and function. This was carried out using DNA, RNA and protein co-extraction. The microbial structure of each fraction was examined using 16S rRNA deep sequencing, while the active members of the consortia were identified using the same approach on cDNA generated from co-extracted RNA samples. Microbial function was investigated using a metaproteomic workflow combining SDS-PAGE and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2017-12-07
AnnouncementXML	Submission_2017-12-07_05:50:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD007956
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aoife Joyce
SpeciesList	scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854;
ModificationList	Oxidation; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-12 03:52:42	ID requested
⏵ 1	2017-12-07 05:50:15	announced
2	2019-03-07 05:42:11	announced	Updated publication reference for PubMed record(s): 29619022.

Dataset with its publication pending

curator keyword: Metaproteomics

submitter keyword: Anaerobic digestion, Metaproteomics, Microbiome, 16S Illumina

Florence Abram
contact affiliation	Functional Environmental Microbiology, School of Natural S iences, National University of Ireland, Galway
contact email	florence.abram@nuigalway.ie
lab head
Aoife Joyce
contact affiliation	NUI Maynooth
contact email	feefeeseoighe@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD007956
     1. Label: PRIDE project
     2. Name: Linking microbial community structure and function during the anaerobic digestion of grass