PXD007956-2

PXD007956 is an original dataset announced via ProteomeXchange.

Title	Linking microbial community structure and function during the anaerobic digestion of grass
Description	In this study, microbial communities from triplicate leach-bed anaerobic bioreactors digesting grass were analysed. Each reactor comprised two microbial fractions, one immobilized on grass (biofilm) and the other in a planktonic state present in the leachate. Microbial communities from the two fractions were systematically investigated for community composition and function. This was carried out using DNA, RNA and protein co-extraction. The microbial structure of each fraction was examined using 16S rRNA deep sequencing, while the active members of the consortia were identified using the same approach on cDNA generated from co-extracted RNA samples. Microbial function was investigated using a metaproteomic workflow combining SDS-PAGE and LC-MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2018-10-23
AnnouncementXML	Submission_2019-03-07_05:42:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aoife Joyce
SpeciesList	scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-10-12 03:52:42	ID requested
1	2017-12-07 05:50:15	announced
⏵ 2	2019-03-07 05:42:11	announced	Updated publication reference for PubMed record(s): 29619022.

Joyce A, Ijaz UZ, Nzeteu C, Vaughan A, Shirran SL, Botting CH, Quince C, O'Flaherty V, Abram F, Linking Microbial Community Structure and Function During the Acidified Anaerobic Digestion of Grass. Front Microbiol, 9():540(2018) [pubmed]

curator keyword: Biological

submitter keyword: Anaerobic digestion

Metaproteomics

Grass

Florence Abram
contact affiliation	Functional Environmental Microbiology, School of Natural Sciences, National University of Ireland Galway
contact email	florence.abram@nuigalway.ie
lab head
Aoife Joyce
contact affiliation	NUI Maynooth
contact email	feefeeseoighe@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD007956

PRIDE project URI

• PRIDE
  1. PXD007956
     1. Label: PRIDE project
     2. Name: Linking microbial community structure and function during the anaerobic digestion of grass