<<< Full experiment listing


PXD010515 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTrichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean
DescriptionNitrogen fixation by cyanobacteria supplies critical bioavailable nitrogen to marine ecosystems worldwide; however, field and lab data have demonstrated it to be limited by iron, phosphorus and/or CO2. To address unknown future interactions among these factors, we grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. Fe/P co-limited cell lines demonstrated a complex cellular response including increased growth rates, broad proteome restructuring and cell size reductions relative to steady-state growth limited by either Fe or P alone. Fe/P co-limitation increased abundance of a protein containing a conserved domain previously implicated in cell size regulation, suggesting a similar role in Trichodesmium. Increased CO2 further induced nutrient-limited proteome shifts in widespread core metabolisms. Our results thus suggest that N2-fixing microbes may be significantly impacted by interactions between elevated CO2 and nutrient limitation, with broad implications for global biogeochemical cycles in the future ocean. Sample number sample description 1 380-1 2 380-2 3 380-3 4 750-1 5 750-2 6 750-3 7 380-1P 8 380-2P 9 380-3P 10 800-1P 11 800-2P 12 800-3P 13 380-1Fe 14 380-2Fe 15 380-3Fe 16 750-1Fe 17 750-2Fe 18 750-3Fe 19 380-1FeP 20 380-2FeP 21 380-3FeP 22 750-1FeP 23 750-2FeP 24 750-3FeP
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew McIlvin
SpeciesList scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-20 04:16:25ID requested
12018-08-09 09:40:07announced
22018-12-04 01:41:31announcedUpdated project metadata.
32021-12-07 02:43:19announced2021-12-07: Updated publication reference for PubMed record(s): 27346420, 34855411.
Publication List
Walworth NG, Saito MA, Lee MD, McIlvin MR, Moran DM, Kellogg RM, Fu FX, Hutchins DA, Webb EA, . J Proteome Res, 21(1):77-89(2022) [pubmed]
Walworth NG, Fu FX, Webb EA, Saito MA, Moran D, Mcllvin MR, Lee MD, Hutchins DA, Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nat Commun, 7():12081(2016) [pubmed]
Keyword List
curator keyword: Metaproteomics
submitter keyword: Trichodesmium, ocean marine microbes, iron and phosphorus co-limitation, Thermo Fusion
Contact List
Mak Saito
contact affiliationMarine Chemistry/Geochemistry Woods Hole Oceanographic Institution USA
contact emailmsaito@whoi.edu
lab head
Matthew McIlvin
contact affiliationWoods Hole Oceanographic Inst.
contact emailmmcilvin@whoi.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010515
PRIDE project URI
Repository Record List
[ + ]