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PXD010515

PXD010515 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTrichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean
DescriptionNitrogen fixation by cyanobacteria supplies critical bioavailable nitrogen to marine ecosystems worldwide; however, field and lab data have demonstrated it to be limited by iron, phosphorus and/or CO2. To address unknown future interactions among these factors, we grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. Fe/P co-limited cell lines demonstrated a complex cellular response including increased growth rates, broad proteome restructuring and cell size reductions relative to steady-state growth limited by either Fe or P alone. Fe/P co-limitation increased abundance of a protein containing a conserved domain previously implicated in cell size regulation, suggesting a similar role in Trichodesmium. Increased CO2 further induced nutrient-limited proteome shifts in widespread core metabolisms. Our results thus suggest that N2-fixing microbes may be significantly impacted by interactions between elevated CO2 and nutrient limitation, with broad implications for global biogeochemical cycles in the future ocean. Sample number sample description 1 380-1 2 380-2 3 380-3 4 750-1 5 750-2 6 750-3 7 380-1P 8 380-2P 9 380-3P 10 800-1P 11 800-2P 12 800-3P 13 380-1Fe 14 380-2Fe 15 380-3Fe 16 750-1Fe 17 750-2Fe 18 750-3Fe 19 380-1FeP 20 380-2FeP 21 380-3FeP 22 750-1FeP 23 750-2FeP 24 750-3FeP
HostingRepositoryPRIDE
AnnounceDate2021-12-07
AnnouncementXMLSubmission_2021-12-07_02:43:18.752.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD010515
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew McIlvin
SpeciesList scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-07-20 04:16:25ID requested
12018-08-09 09:40:07announced
22018-12-04 01:41:31announcedUpdated project metadata.
32021-12-07 02:43:19announced2021-12-07: Updated publication reference for PubMed record(s): 27346420, 34855411.
Publication List
Walworth NG, Saito MA, Lee MD, McIlvin MR, Moran DM, Kellogg RM, Fu FX, Hutchins DA, Webb EA, . J Proteome Res, 21(1):77-89(2022) [pubmed]
Walworth NG, Fu FX, Webb EA, Saito MA, Moran D, Mcllvin MR, Lee MD, Hutchins DA, Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nat Commun, 7():12081(2016) [pubmed]
Keyword List
curator keyword: Metaproteomics
submitter keyword: Trichodesmium, ocean marine microbes, iron and phosphorus co-limitation, Thermo Fusion
Contact List
Mak Saito
contact affiliationMarine Chemistry/Geochemistry Woods Hole Oceanographic Institution USA
contact emailmsaito@whoi.edu
lab head
Matthew McIlvin
contact affiliationWoods Hole Oceanographic Inst.
contact emailmmcilvin@whoi.edu
dataset submitter
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Dataset FTP location
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