PXD010515-3

PXD010515 is an original dataset announced via ProteomeXchange.

Title	Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean
Description	Nitrogen fixation by cyanobacteria supplies critical bioavailable nitrogen to marine ecosystems worldwide; however, field and lab data have demonstrated it to be limited by iron, phosphorus and/or CO2. To address unknown future interactions among these factors, we grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. Fe/P co-limited cell lines demonstrated a complex cellular response including increased growth rates, broad proteome restructuring and cell size reductions relative to steady-state growth limited by either Fe or P alone. Fe/P co-limitation increased abundance of a protein containing a conserved domain previously implicated in cell size regulation, suggesting a similar role in Trichodesmium. Increased CO2 further induced nutrient-limited proteome shifts in widespread core metabolisms. Our results thus suggest that N2-fixing microbes may be significantly impacted by interactions between elevated CO2 and nutrient limitation, with broad implications for global biogeochemical cycles in the future ocean. Sample number sample description 1 380-1 2 380-2 3 380-3 4 750-1 5 750-2 6 750-3 7 380-1P 8 380-2P 9 380-3P 10 800-1P 11 800-2P 12 800-3P 13 380-1Fe 14 380-2Fe 15 380-3Fe 16 750-1Fe 17 750-2Fe 18 750-3Fe 19 380-1FeP 20 380-2FeP 21 380-3FeP 22 750-1FeP 23 750-2FeP 24 750-3FeP
HostingRepository	PRIDE
AnnounceDate	2021-12-07
AnnouncementXML	Submission_2021-12-07_02:43:18.752.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 04:16:25	ID requested
1	2018-08-09 09:40:07	announced
2	2018-12-04 01:41:31	announced	Updated project metadata.
⏵ 3	2021-12-07 02:43:19	announced	2021-12-07: Updated publication reference for PubMed record(s): 27346420, 34855411.

Walworth NG, Saito MA, Lee MD, McIlvin MR, Moran DM, Kellogg RM, Fu FX, Hutchins DA, Webb EA, . J Proteome Res, 21(1):77-89(2022) [pubmed]

Walworth NG, Fu FX, Webb EA, Saito MA, Moran D, Mcllvin MR, Lee MD, Hutchins DA, Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nat Commun, 7():12081(2016) [pubmed]

curator keyword: Metaproteomics

submitter keyword: Trichodesmium, ocean marine microbes, iron and phosphorus co-limitation, Thermo Fusion

Mak Saito
contact affiliation	Marine Chemistry/Geochemistry Woods Hole Oceanographic Institution USA
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD010515

PRIDE project URI

• PRIDE
  1. PXD010515
     1. Label: PRIDE project
     2. Name: Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean