PXD010515-4

PXD010515 is an original dataset announced via ProteomeXchange.

Title	Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean
Description	Nitrogen fixation by cyanobacteria supplies critical bioavailable nitrogen to marine ecosystems worldwide; however, field and lab data have demonstrated it to be limited by iron, phosphorus and/or CO2. To address unknown future interactions among these factors, we grew the nitrogen-fixing cyanobacterium Trichodesmium for 1 year under Fe/P co-limitation following 7 years of both low and high CO2 selection. Fe/P co-limited cell lines demonstrated a complex cellular response including increased growth rates, broad proteome restructuring and cell size reductions relative to steady-state growth limited by either Fe or P alone. Fe/P co-limitation increased abundance of a protein containing a conserved domain previously implicated in cell size regulation, suggesting a similar role in Trichodesmium. Increased CO2 further induced nutrient-limited proteome shifts in widespread core metabolisms. Our results thus suggest that N2-fixing microbes may be significantly impacted by interactions between elevated CO2 and nutrient limitation, with broad implications for global biogeochemical cycles in the future ocean. Sample number sample description 1 380-1 2 380-2 3 380-3 4 750-1 5 750-2 6 750-3 7 380-1P 8 380-2P 9 380-3P 10 800-1P 11 800-2P 12 800-3P 13 380-1Fe 14 380-2Fe 15 380-3Fe 16 750-1Fe 17 750-2Fe 18 750-3Fe 19 380-1FeP 20 380-2FeP 21 380-3FeP 22 750-1FeP 23 750-2FeP 24 750-3FeP
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:34.207.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Matthew McIlvin
SpeciesList	scientific name: Trichodesmium erythraeum IMS101; NCBI TaxID: 203124;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-07-20 04:16:25	ID requested
1	2018-08-09 09:40:07	announced
2	2018-12-04 01:41:31	announced	Updated project metadata.
3	2021-12-07 02:43:19	announced	2021-12-07: Updated publication reference for PubMed record(s): 27346420, 34855411.
⏵ 4	2024-10-22 04:46:39	announced	2024-10-22: Updated project metadata.

10.1038/ncomms12081;

Walworth NG, Saito MA, Lee MD, McIlvin MR, Moran DM, Kellogg RM, Fu FX, Hutchins DA, Webb EA, . J Proteome Res, 21(1):77-89(2022) [pubmed]

Walworth NG, Fu FX, Webb EA, Saito MA, Moran D, Mcllvin MR, Lee MD, Hutchins DA, Mechanisms of increased Trichodesmium fitness under iron and phosphorus co-limitation in the present and future ocean. Nat Commun, 7():12081(2016) [pubmed]

10.1021/acs.jproteome.1c00517;

10.6019/PXD010515;

curator keyword: Metaproteomics

submitter keyword: ocean marine microbes, Thermo Fusion,Trichodesmium, iron and phosphorus co-limitation

Mak Saito
contact affiliation	Marine Chemistry/Geochemistry Woods Hole Oceanographic Institution USA
contact email	msaito@whoi.edu
lab head
Matthew McIlvin
contact affiliation	Woods Hole Oceanographic Inst.
contact email	mmcilvin@whoi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010515
     1. Label: PRIDE project
     2. Name: Trichodesmium LC-MSMS iron and phosphorous co-limitation in the ocean